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Barabash, Y.
Holographic interferometry as a method to study conformational changes in macromolecules [] !Otitkn.pft: FILE NOT FOUND! !oizd.pft: FILE NOT FOUND! !ospec.pft: FILE NOT FOUND! !oistaspk_H.pft: FILE NOT FOUND! Рубрикатор НБУВ: Тематичні рубрики:
Шифр журнала:
Кл.слова (ненормированные):
photoinduced change -- electron transfer -- holographic interferometer -- photo-thermoplastical registering media -- refraction index
Анотація: This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (<$E DELTA n>) influenced by the sample volume expansion due to temperature changes (<$E DELTA n sub T>), light absorption (<$E DELTA n sub abs>) and changes in the volume of photoactive molecules (<$E DELTA n sub V>). Using the scheme of temperature compensation we could reduce the task to the case <$E DELTA n sub T ~<<<<~ DELTA n sub abs ,~ DELTA n sub V>. At RC concentrations close to <$E 10 sup 15 ~roman cm sup -3>, our experimental setup enabled to register the shift of an interference pattern up to 0,1 of the bandwidth. Our estimations for the case of a protein solution yields in relative changes in the molecular volume within <$E 10 sup -3 ~-~ 10 sup -2>. !oprip481_H.pft: FILE NOT FOUND!
Дод. точки доступу:
Kharkyanen, V.; Zabolotny, M.; Sokolov, N.
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