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Список видань за алфавітом назв: Авторський покажчик Покажчик назв публікацій  |
Пошуковий запит: (<.>A=Platonov O$<.>) | |||
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Загальна кількість знайдених документів : 9 Представлено документи з 1 до 9 |
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| 1. | 
Platonov O. Рreconditions for economic security of supply chains of businesses in multimodal transportation [Електронний ресурс] / O. Platonov // Збірник наукових праць Державного економіко-технологічного університету транспорту. Сер. : Економіка і управління. - 2015. - Вип. 33. - С. 54-61. - Режим доступу: http://nbuv.gov.ua/UJRN/Znpdetut_eiu_2015_33_8 | ||
| 2. |
Platonov O. The economic security mechanism for organizations in multimodal transportation system [Електронний ресурс] / O. Platonov // Збірник наукових праць Державного економіко-технологічного університету транспорту. Сер. : Економіка і управління. - 2015. - Вип. 34. - С. 35-43. - Режим доступу: http://nbuv.gov.ua/UJRN/Znpdetut_eiu_2015_34_6 | ||
| 3. | 
Platonov O. I. State financial support prospects for inland waterways upgrading to include the river transport in a multimodal transport system [Електронний ресурс] / O. I. Platonov // Public manаgement. - 2019. - № 4. - С. 200-210. - Режим доступу: http://nbuv.gov.ua/UJRN/pubm_2019_4_18 | ||
| 4. | 
Iskandarov E. Action of venom of Vipera snake of Ukraine on blood coagulation in vitro [Електронний ресурс] / E. Iskandarov, O. Zinenko, A. Tupikov, A. Pitishkina, O. Platonov, V. Gryshchuk, Y. Kucheriavyi, Y. Stohnii // Biotechnologia Acta. - 2022. - Vol. 15, № 2. - С. 56-57. - Режим доступу: http://nbuv.gov.ua/UJRN/biot_2022_15_2_10 Aim. In this study we focused on the search of fibrinogen-targeted proteases in the venom of Vipera renardi, Vipera nikolskii and Vipera berus. Venom of Vipera berus was also fractionated on Q-sepharose and action of separated fractions on human blood plasma, platelets and red cells was studied. Methods. Analysis of protein mixtures was performed using SDS-PAGE. Action on the blood coagulation system was analyzed using the APTT assay. Identification of protein components with fibrinolytic activity was performed using enzyme-electrophoresis with fibrinogen as the substrate. Fractionation of V. berus venom was performed on Q-sepharose using FPLC system Acta Prime. Action of separated fractions on ADP-induced platelet aggregation in platelet rich blood plasma was analyzed using Aggregometer AP 2110. Hemolytic action of fractions was estimated using fresh human red cells. Amount of released hemoglobin was estimated by spectrophotometry on Optizen POP. Results. All studied venoms had different protein compositions with major protein fractions in the range from 25 kDa to 130 kDa. Both V. berus and V. nikolskii venoms taken in 1:200 dilutions reduced the time of clotting in APTT test from 25 to 13 s. In contrast, V. renardi venom in the same dilution prolonged the clotting time from 25 s to 180 s that we assumed as the result of fibrinogen-specific protease presence. According to enzyme-electrophoresis data all studied venoms contained fibrinogen-specific proteases with the apparent molecular weights for V. berus, V. nikolskii - 25 - 55 kDa. and V. renardi - 55 - 75 kDa. Fractionation of crude venom of V. berus allowed obtaining several fractions eluted at different concentrations of NaCl: 0,1; 0,2; 0,3; 0,5 M. Non-binded fraction was also collected. Conclusions. Thus, the components of Vipera venoms living in Ukraine can be used for basic biochemical research. At the same time, care should be taken in the case of envenomation, as the presence of fibrinogenolytic enzymes in the venom can lead to hemorrhage. Further characterization of fibrinogen-specific protease from V. berus venom is a promising task for biotechnology.Aim - in this study we focused on the search of fibrinogen-targeted proteases in the venom of Vipera lebetina. Methods - fractionation of the venom was performed using FPLC chromatographic system Acta Prime on Q Sepharose. Analysis of protein mixtures was performed using SDS-PAGE. Аction on blood coagulation system was analyzed using APTT assay [2]. Proteolytic action on fibrinogen and identification of protein components with fibrinolytic activity was performed using electrophoresis of mixture of fibrinogen solution (2 mg/ml) with venom's fractions. For a comprehensive evaluation of the effect of the obtained fractions on hemostasis, an original approach with modified aggregatometry was used [3]. This approach made it possible to take into account the ability of fractions to activate platelets, initiate blood coagulation, or inhibit platelet aggregation. Hemolytic action of fractions was estimated using fresh human red cells. Amount of released hemoglobin was estimated by spectrophotometry on Optizen POP. Crude venom of V. lebetina was fractionated using ion-exchange chromatography on Q Sepharose. Elution was performed using a stepwise gradient of NaCl (0,1, 0,2, 0,3, 0,5, and 0,7 M NaCl) in 0,05 M Tris-HCl buffer at pH 8,3. Fractions eluted at 0,1 and 0,2 M of NaCl contained several proteins with different molecular weights ranging from 75 kDa to low molecular weight fractions according to the SDS-PAGE. Proteins that cleave <$Ealpha>- and <$Ebeta>-chains of fibrinogen were found in fractions 0,1 and 0,2, indicating the presence of an enzyme with fibrinogenolytic activity in the venom of V. lebetina. The fractions 0,3, 0,5, and 0,7 did not show any significant fibrinogenolytic activity. After platelet aggregation study we concluded that fraction 0,1 contained a protein with fibrinogenolytic activity. An increase in platelet aggregation was observed for the fraction 0,2 after the addition of ADP. This may indicate the presence of an active compound that promotes platelet aggregation. Further research is necessary to determine its nature. Fractions 0,3, 0,5, and 0,7 had no effect on platelet aggregation. A decrease in blood plasma clotting time in APTT to 5 s and 7 s, compared to a control value of 70 s, was shown for fractions eluted at NaCl concentrations of 0,1 M and 0,2 M, respectively. The fractions 0,3, 0,5 had only a slight effect on reducing blood plasma clotting. A slightly increased level of hemolysis was shown in the presence of the unbound fraction and the whole venom. It can be suggested that proteins with phospholipase activity are present in the non-binded fraction. Conclusions: thus, fibrinogen-specific proteases, hemolytic agents, activators of blood clotting were found in the venom of Vipera lebetina. Most of these compounds must to be purified and can be used for basic biochemical research. | ||
| 5. |
Baidakova K. V. Fibrinogen-specific protease in the Vipera renardi snake venom [Електронний ресурс] / K. V. Baidakova, Yе. M. Stohnii, O. M. Platonov // Biotechnologia Acta. - 2023. - Vol. 16, № 2. - С. 11-12. - Режим доступу: http://nbuv.gov.ua/UJRN/biot_2023_16_2_4 Aim - to search fibrinogenolytic enzymes among protein components of Vipera renardi snake venom. Methods - Venom of V. renardi as the lyophilized powder was supplied by Trypillia serpentarium. It was dissolved in 0,05 M Tris-HCl buffer pH 8,3 and fractionated on Superdex G-75 using FPLC system Acta Prime. Peaks were tested for their ability to directly cleave fibrinogen. Hydrolytic products were analyzed by SDS-PAGE. Enzyme-electrophoresis with fibrinogen co-polymerized in 12 % polyacrylamide gel was used for the identification of protein that can cleave fibrinogen. Venom of V. renardi was fractionated on 4 fractions using size-exclusion chromatography. SDS-PAGE of fibrinogen hydrolysis products showed the presence of fibrinogen-specific protease in the 1st and 2nd fractions of venom. 2<^>nd | ||
| 6. |
Platonov O. M. Ex vivo Study of the action of integrin receptors [Електронний ресурс] / O. M. Platonov, I. V. Us // Biotechnologia Acta. - 2023. - Vol. 16, № 2. - С. 37-39. - Режим доступу: http://nbuv.gov.ua/UJRN/biot_2023_16_2_14 Aim - in our work, we studied platelet aggregation in blood plasma of pregnant women and estimated the possibility of ex vivo normalization of aggregation rate using a polypeptide from the Echis multisquamatus snake venom. Previous reports demonstrated that it directly interacts with glycoprotein IIb/IIIa receptors on the surface of platelets, preventing their adhesion, thereby affecting the degree of aggregation. Method - chromatography followed by size-exclusion chromatography on Superdex 75 using the FPLC system (AKTA, GE Healthcare, USA). Analysis of molecular weight of protein components was performed using SDS-PAGE. The concentration of protein was measured using spectrophotometer Optizen POP (Korea) at 280 nm. The ability of obtained protein to inhibit platelet aggregation was measured directly by aggregometry. Blood samples of women with placental disfunction during pregnancy (n = 28) were kindly provided by "Perinatal Center of Kyiv". This study was approved by the Ethics Commission of the Shupyk National Medical Academy of Postgraduate Education and the Ethics Commission of the Kyiv Perinatal Center (<35> 3 from 05/05/2020). Aggregation of platelet-rich plasma (PRP) induced by ADP was investigated using aggregometry on the AP 2110 (Solar, Belarus). We compared the rate of platelet aggregation in the presence vs absence of platelet aggregation inhibitor. Two-step chromatography protocol allowed us to obtain the polypeptide from the venom of Echis multisquamatus that possessed the anti-aggregatory action. SDS-PAGE analysis confirmed the homogeneity of obtained polypeptide with apparent molecular weight 14 kDa that corresponds to the platelet aggregation inhibitor reported earlier. Initial studies of ADP-induced platelet aggregation allowed selecting active concentration for the effective inhibitory action as 0,02 mg/ml. Conclusions: platelet aggregation inhibitor from Echis multisquamatis snake venom of can be assumed as the effective agent that reduce the rate of platelet aggregation. We demonstrated it efficacy in platelet rich plasma of pregnant women that had placenta dysfunction. The use of direct antagonist of platelet integrin receptors was assumed as the prospective approach for suppressing of platelet reactivity in particular during complicated pregnancy. | ||
| 7. | 
Korolova D. S. Thromboelastographic study of fibrin clot and molecular basis of maximum clot firmness [Електронний ресурс] / D. S. Korolova, Y. M. Stohnii, V. I. Gryshchuk, S. I. Zhuk, I. V. Us, T. M. Chernyshenko, O. P. Kostiuchenko, K. P. Klymenko, O. M. Platonov, O. I. Ivashchenko, V. O. Chernyshenko // The Ukrainian Biochemical Journal. - 2021. - Vol. 93, № 2. - С. 62-70. - Режим доступу: http://nbuv.gov.ua/UJRN/BioChem_2021_93_2_8 | ||
| 8. |
Platonov O. Purification and characterization of platelet aggregation inhibitor from the venom of Bitis arietans [Електронний ресурс] / O. Platonov, V. Nikulina, Y. Kucheryavyi, V. Gryshchuk, Y. Stohniy, V. Chernyshenko, O. Slominskyi, A. Rebriev, K. Savchenko, L. Garmanchuk // The Ukrainian Biochemical Journal. - 2022. - Vol. 94, № 5. - С. 7-17. - Режим доступу: http://nbuv.gov.ua/UJRN/BioChem_2022_94_5_4 | ||
| 9. |
Platonov O. I. Unification of the variety of interpretations of basic determinants of the concept "mechanism of state regulation of multimodal transport of goods" [Електронний ресурс] / O. I. Platonov // Public manаgement. - 2020. - № 4. - С. 252-268. - Режим доступу: http://nbuv.gov.ua/UJRN/pubm_2020_4_22 | ||
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