The article has investigated the removal of water borne pathogens using dielectrophoresis (DEP) filter which is energized by varying the frequency of the applied potential from 10 kHz to 2 MHz with different voltage levels of 5; 10; 15 and 20 V. Separate experiments are conducted in artificially contaminated water samples with Escherichia coli, Staphylococcus aureus and Vibrio cholera up to 2 h. The impact of signal frequency and voltages on DEP based water treatment system has been analyzed statistically. Results have demonstrated that an ac signal of 20 V with frequency range of 500 kHz to 2 MHZ is suitable to remove the tested bacterial population and the rate of removal of E. coli is the highest with a dielectrophoretic filtration efficiency of 77,1 %.