The effects of a biotechnological probiotic product, PP, produced by food fermentation with Lactobacilli (US patent approved), on the growth of neurites in rat pheochromocytoma cells (PC-12) and on calcium responses of rat brain neurons were studied in culture. The PP increased the length of neurites in PC-12 cells, resulting in an irreversible differentiation of cancerous cells into neuron-like structures. Moreover, a change in the neurotransmitter phenotype of differentiated cells was found; some cells, such as excitatory neurons, began to respond to glutamate application by increasing <$E [ roman Ca sup 2+ ] sub i>. The PP directly activated PC-12 cells and neurons by the release of <$E roman Ca sup 2+> from the intracellular stores in a steady manner. The PP also stimulated the entry of <$E roman Ca sup 2+> into the cells in a <$E roman Ca sup 2+> add-back protocol, which was considerable upon washing out of PP. Thus, the products of Lactobacillus metabolism, such as those in PP, can act as a neuronal growth factor and manifest clear pharmacological reactions at the cellular level. By comparison, commercial lyophilized probiotic bacteria also induced a <$E roman Ca sup 2+> rise in neurons, but not in PC-12 cells. Some neurons did not respond to probiotic bacteria, and some neurons responded with some delay. Upon wash out of probiotic bacteria, a huge entry of <$E roman Ca sup 2+> into the cells was observed.

Ключ. слова: probiotics, neurite outgrowth, PC-12 differentiation, calcium signalling, rat brain neurons
Індекс рубрикатора НБУВ: Е60*739.177 + Р281.794-2

Рубрики:

Загальна зоологія

Біогенні стимулятори, адаптогени

Шифр НБУВ: Ж61875 Пошук видання у каталогах НБУВ 

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