The recombinant coat protein of Alfalfa Mosaic Virus in the bacterial expression system is obtained through clonning ORF of the CP gene into plasmid pET24a using the PCR technique. The protein had some amino acidic replacements and on three amino acidic residuals was the shortly original protein. Immunization of rabbits was done with 0,5 mg of respective purified recombinant CP emulsified in Freund's complete adjuvant. ELISA test and Western blotting were performed. The results have shown that antiserum reacted strongly with the protein whose apparent molecular mass was near 30~kD in Western blot analysis and the titer of antibodies composed 1/3200 (OD492 1,3) in the ELISA reaction. The necessity of antiserum for immunoassays on the screening of transgenic plants and for estimating the level of expression of the AMV coat protein by host plants is shown.

• Мельничук Максим Дмитрович (1973–) (біологічні науки)
• Мельничук Микола Дмитрович (технічні науки)

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