The influence of transmembrane proton gradient (<$E DELTA roman pH>) on the contribution of photo system II (PSII) and photosystem I (PSI) in the ferricyanide (FeCy) photoreduction in isolated chloroplasts is examined. The <$E DELTA roman pH> level is measured by fluorescence quenching of 9-aminoacridine and modulated by the uncoupler <$E roman {NH sub 4 Cl}> or by phosphorylation substrates and ATP. To estimate the contribution of PSI and PSII to the FeCy photoreduction, we used DBMIB and NQNO which are inhibitors of the cytochrome <$E b sub 6 f>-complex. It is show that the level of electron transfer from water to FeCy depends on the addition of adenine nucleotides and is correlated with <$E DELTA roman pH> changes. The PSII-dependent part of the FeCy photoreduction was decreased in the presence of phosphorylation substrates or relatively low concentrations of the uncoupler <$E roman {NH sub 4 Cl}>, which decreases the <$E DELTA roman pH> level. With increasing <$E DELTA roman pH> induced by ATP, the PSII contribution to the FeCy photoreduction was increased. We may propose that the accessibility of the acceptor site of PSII depends on the transthylakoid <$E DELTA roman pH>.

• Онойко Олена Борисівна (біологічні науки)
• Подорванов Володимир Вікторович (1960–2020) (біологічні науки)
• Золотарьова Олена Костянтинівна (1952–) (біологічні науки)

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