Індекс рубрикатора НБУВ: Р569.60-5

Рубрики:

Пухлини шкіри та слизових оболонок

Шифр НБУВ: Ж14160 Пошук видання у каталогах НБУВ 

Індекс рубрикатора НБУВ: Р569.423-5

Рубрики:

Пухлини легенів

Шифр НБУВ: Ж14160 Пошук видання у каталогах НБУВ 

Aim - to investigate the anticancer efficiency of the xenogeneic vaccine in different tumor models and to assess the possibility whether level of antibodies (Ab) specific for vaccine's proteins can be used as an indication for its use. Mice with Lewis lung carcinoma (LLC), Ehrlich carcinoma (EC) or Sarcoma 37 (S37) were immunized with a xenogeneic anticancer vaccine based on chicken embryo proteins (CEP) and its anticancer activity was examined. The level of specific Ab in the blood serum of non-immunized tumor-bearing mice was studied by ELISA. CEP application statically significantly inhibited the growth of LLC (the index of tumor growth inhibition was 42,10 - 53,13 % depending on the day of tumor growth); vaccinated mice with EC showed significant tumor growth inhibition and life prolongation by 34,48 %. Among mice with S37, there was noticed no antitumor effect. The number of tumorbearing nonimmunized mice which have had preexisting CEP-specific Ab did not differ depending on the tumor model. The level of CEP-specific Ab among mice with LLC and EC increased with the growth of the tumor volume, but it decreased among mice bearing S37. Probably, the low level of CEP-specific Ab alongside huge tumor burden shows it is futile to apply the CEP-based vaccine. Conclusion: different tumor strains vary in their susceptibility to CEP-based vaccine. Probably, the low level of CEP-specific Ab when a tumor burden is huge shows it is futile to apply the CEP-based vaccine.

Aim - to investigate anticancer and immunologic effects of chicken embryonic proteins (CEP) in mice bearing Ehrlich solid carcinoma. The study was carried out on male Balb/c mice bearing Ehrlich solid carcinoma. The immunizations were performed after the tumor transplantation. The immune status was assessed on days 7, 14, 21 and 28 after the tumor challenge. Cytotoxic activity (CAT) of macrophages (Mph), natural killer cells (NK), cytotoxic T-lymphocytes (CTL) and blood serum, as well as the influence of the blood serum on immune cells activity was checked in MTT-assay; Mph's cytochemical activity was tested in NBT-assay; Ehrlich antigen-specific or CEP-specific antibodies were detected in ELISA-assay; medium size circulating immune complexes (CIC) were detected in reaction of 4,5 % polyethylene glycol precipitation. The immunization resulted in tumor growth suppression and significant 25,64 % prolongation of the survival time. In both control and immunized mice with transplanted tumors antibodies specific to Ehrlich carcinoma antigens and to CEP were detected, but antibody response was more balanced in the treatment group. In the treatment group both cytochemical and CAT of Mph was moderately activated and well preserved until late stages of tumor development; CAT of NK and CTL remained in the range of the intact mice until day 28 after the tumor transplantation. The immunized mice were well protected from accumulation of CIC and suppressive activity of autologous blood serum. Conclusion: collectively, our data indicate that CEP can elicit immunomodulating and immunoprotecting effects sufficient to provide tumor growth inhibition. The further elaboration of a xenogeneic anticancer vaccine based on CEP is warranted.

Aim - to investigate the effect of chicken embryo proteins (CEP) as a prototype of xenogeneic vaccine on immune reactions in mice immunized after Lewis lung carcinoma (LLC) surgical removal. C57Bl male mice were immunized on days 1, 8, and 15 after surgical removal of LLC. The immune response was assessed on days 7, 14, 21 and 28 after tumor resection. Cytotoxic activity of natural killer cells (NK) and cytotoxic T-lymphocytes as well as antibody dependent cellular cytotoxicity was estimated in MTT-assay; specific antibodies were detected in ELISA; lymphocyte proliferation was tested in reaction of in vitro blast transformation. None of the immunized mice developed LLC metastases. Immunization with CEP seems to prevent the potential decrease in NK cell cytotoxic activity and spontaneous blast transformation activity of lymphocytes following the surgically induced stress. Further research on improving immunization schedule and elucidating the mechanisms of NK modulation with CEP is needed.

Aim - the purpose of the study was to evaluate the effect of B. subtilis IMV B-7724 lectin on the functional activity of macrophages (Mph) and natural killer cells (NK) of intact Balb/c mice. Balb/c mice were subjected to 10 consecutive administrations of the lectin in a dose of 1 mg/kg of body weight. The functional activity of peritoneal Mph and NK were studied. Statistical analysis of the results was performed according to the widely accepted methods of variational statistics. Administration of bacterial lectin increased Mph and NK cytotoxic activity; maximal increase was registered after the complete course of injections. A significant increase in the NO production indicates the polarization of peritoneal Mph into proinflammatory type M1. The transcription factors of IRF (at the early stage) and STAT (at the latter stages) signalling pathways were involved in the process of Mph polarization. Conclusion: the ability of B. subtillis IMV B-7724 lectin to increase in vivo cytotoxic activity of innate immunity effectors and to maintain the long-term Mph M1 polarization urges further studies on the lectin effectiveness.

Шифр НБУВ: Ж100178 Пошук видання у каталогах НБУВ